|
| ||
| Component | Amount per reaction | Final concentration |
|
| ||
| Recovered plasmid DNA (Step 20) | Variable | 30 ng/μl |
| NEB buffer 2 (10×) | 1 μl | 1× |
| EcoRI (20 U/μl) | 0.25 μl | 0.5 U/μl |
| SAP (1 U/μl) | 1 μl | 0.1 U/μl |
| Sterile deionized water | Variable | — |
| Total volume | 10 μl | — |
|
| ||
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