|
| ||
| Component | Amount per reaction (μl) | Final concentration |
|
| ||
| Reaction mixture (Step 22) | 15 | — |
| NEB buffer 2 (10×) | 0.5 | 1× |
| BSA (100×) | 0.2 | 1× |
| NheI (10 U/μl) | 0.5 | 0.25 U/μl |
| Sterile deionized water | 3.8 | — |
| Total volume | 20 | — |
|
| ||
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