. Author manuscript; available in PMC: 2016 Jan 7.

Published in final edited form as: J Proteome Res. 2008 Apr 15;7(5):1904–1915. doi: 10.1021/pr700651b

Table 1. A List of Biotinylated Proteins and Their Peptides Identified from WM-266-4 Cells Used To Evaluate the Quantification Strategy Based on Cell Surface Biotinylation and Affinity-Enriched Peptides^a.

NCBI accession	protein name	peptide used for quantification	charge	m/z	ratio (L/H) 1:1	ratio (L/H) 3:1	ratio (L/H) 10:1
NP_006491	melanoma cell adhesion molecule	R.IFLCQGK@R.P	2	684.9	1.12	3.14	10.35
		R.IFLCQGK#R.P	2	680.9
		R.LPSGNHMK@ESR.E	3	534.9	0.99	3.18	9.91
		R.LPSGNHMK#ESR.E	3	532.3
NP_001001390	CD44 antigen isoform 3 precursor	R.YVQK@GEYR.T	2	695.3	0.75	2.35	8.85
NP_001001390	CD44 antigen isoform 3 precursor	R.YVQK#GEYR.T	2	691.3
NP_002194	integrin alpha 2 precursor	R.K@YAYSAASGGR.R	2	739.3	0.98	2.95	10.02
NP_002194	integrin alpha 2 precursor	R.K#YAYSAASGGR.R	2	735.3
NP_001670	Na+/K+ -ATPase beta 3 subunit	R.IIGLK@PEGVPR.I	2	763.4	1.04	3.07	10.11
NP_001670	Na+/K+ -ATPase beta 3 subunit	R.IIGLK#PEGVPR.I	2	759.4
NP_005492	integrin alpha 3 isoform b precursor	R.TGAVYLCPLTAHK@DDCER.M	3	818.4	1.18	2.91	10.67
		R.TGAVYLCPLTAHK#DDCER.M	3	815.7
				Mean	1.01	2.93	9.99
				SD	0.15	0.30	0.62
				RSD%	14.74	10.38	6.20

Note: #, biotinylated light (L) lysine; @, biotinylated heavy (H) lysine; cysteine is CAM-modified.

Table 1. A List of Biotinylated Proteins and Their Peptides Identified from WM-266-4 Cells Used To Evaluate the Quantification Strategy Based on Cell Surface Biotinylation and Affinity-Enriched Peptidesa.