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. 2015 Nov 18;5(1):67–74. doi: 10.5966/sctm.2015-0077

Figure 4.

Figure 4.

Ferumoxytol-labeled hESC-CPCs differentiation toward definitive cardiac cell types in vivo. (A): Porcine heart harvested at day 40 showing injection sites of ferumoxytol-labeled and unlabeled cells. (B): Transverse sections of day 40-harvested porcine heart; cell grafts are shown in sections 2 (ferumoxytol labeled) and 4 (unlabeled). (C–E): Stained frozen sections of ferumoxytol-labeled hESC-CPCs in injected hearts from sacrificed pigs (day 40). Immunohistochemical stains depict the expression of DAPI (blue), anti-human mitochondria (red), and cTnC (C), CD31 (D), and CNN1 (E) (green). Scale bars = 50 μm. Abbreviations: cTnC, cardiac troponin C; DAPI, 4′,6-diamidino-2-phenylindole; Hu-mito, human mitochondria.