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. 2015 Nov 16;5(1):106–116. doi: 10.5966/sctm.2015-0081

Figure 5.

Figure 5.

The expression of SG development-related genes (i.e., EDA, EGF, and KGF) were examined in natural SGs, differentiated SGCs (SGCs-mix and SGCs-KGF), and hUC-MSCs using RT-PCR and Western blotting analysis. (A): mRNA levels of EDA, EGF, and KGF expression. Differentiated SGCs (SGCs-KGF and SGCs-mix) from hUC-MSCs expressed EDA, EGF, and KGF mRNA, but hUC-MSCs expressed lower levels of mRNA of EGF and KGF. The expression of β-actin was used as a control. (B): Protein expressions of EDA, EGF, and KGF. Natural SGs and differentiated SGCs (SGCs-KGF and SGCs-mix) from hUC-MSCs expressed proteins of EDA, EGF, and KGF but did not in hUC-MSCs in Western blot analysis. Abbreviations: bp, base pair; hUC-MSC, mesenchymal stem cell derived from human umbilical cord Wharton’s jelly; KGF, keratinocyte growth factor; RT-PCR, reverse-transcriptase polymerase chain reaction; SG, sweat gland; SGC, SGC, sweat gland-like cell.