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. 2015 Nov 19;5(1):95–105. doi: 10.5966/sctm.2015-0139

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Figure 2. — Osteogenic differentiation with hGFs and hiPSCs. (A): Alizarin red staining of hGFs (top) and hiPSCs (bottom) at passages 2, 5, and 10. hGFs showed decreased osteogenic differentiation capability as the cell passage number increased. hiPSCs presented with higher osteogenic differentiation capability and elevated calcified nodules, regardless of the passage number, compared with hGFs. Scale bars = 100 μm. (B): Percentage of positive alizarin red staining showed that hiPSCs had significantly increased osteogenic differentiation capability compared with hGFs. Data are presented as mean ± SD (n = 3). ***, p < .001. Abbreviations: hGFs, human gingival fibroblasts; hiPSCs, human induced pluripotent stem cells; ns, no statistically significant difference (p > .05); P, passage.