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. 2015 Nov 19;5(1):95–105. doi: 10.5966/sctm.2015-0139

Figure 6.

Figure 6.

Cone-beam computed tomography (CBCT) imaging, analysis, and specimens of HCG-111 or HCG-311 alone or combined with hiPSCs subcutaneously transplanted into immunocompromised mice in vivo at 12 weeks. (A): Three-dimensional (3D) images of a mouse with pure scaffold transplants. Based on the CBCT data, 3D image creation of soft tissue (Aa), maximum intensity projection (MIP) full-range visualization (Ab), 3D rendering of materials at mouse bone density (Ac), and bone plus soft tissue (Ad) were fabricated using the Software NNT viewer. Images of 0.5-mm-thick free-cut specimens were generated across the four implants (yellow arrows) individually, at upper left (Ae), upper right (Af), lower right (Ag), and lower left (Ah). The material in (Ae, Af) was HCG-111 and in (Ag, Ah) was HCG-311. (B): 3D imaging of mouse with hiPSC/scaffold complexes. Based on the CBCT data, 3D image creation of soft tissue (Ba), MIP full-range visualization (Bb), 3D rendering of materials at mouse bone density (Bc), and bone plus soft tissue (Bd) were fabricated using the Software NNT viewer. Images of 0.5-mm-thick free-cut specimens were generated across the four implants (yellow arrows) individually, at upper left (Be), upper right (Bf), lower right (Bg), and lower left (Bh). The material in (Be, Bf) was hiPSC/HCG-111 and in (Bg, Bh) was hiPSC/HCG-311. (C): Ct value analysis was based on the bone density measurement tool in Software Mimics, version 10.01 (Materialise NV). Samples cultured in vitro for 12 weeks (n = 3) and transplanted in vivo for 12 weeks (n = 12) were quantitatively calculated, and mouse thigh bone sections (n = 3) were measured as the control. The Ct value of hiPSC/HCG-311 was significantly higher than that of hiPSC/HCG-111 in vivo. Data are presented as mean ± SD; ***, p < .001. (D): Photographs of specimens retrieved from mice at 12 weeks. Scale bars = 10 mm. Abbreviations: HCG-111, nanohydroxyapatite/chitosan/gelatin scaffold with 1 wt/vol% nanohydroxyapatite; HCG-311, nanohydroxyapatite/chitosan/gelatin scaffold with 3 wt/vol% nanohydroxyapatite; hiPSC, human induced pluripotent stem cell; HU, Hounsfield unit.