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. 2016 Jan 7;6(1):16. doi: 10.1007/s13205-015-0334-1

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — Cloning of CAVA gene: a amplified CAVA gene. Lane 1: Marker and Lane 2 is an amplified product of 801 bp. b Confirmed constructed plasmid by colony PCR. Lane 1 is a marker and lanes 3 to 8 are amplified products, further confirmed by restriction digestion. c Digested products back bone of 5.4 kb of pET21c and gene of CAVA of 801 nucleotides (Lane 2) and Lane 1 is a marker