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Purification of CAVA under native condition. a SDS-PAGE showing the expression of recombinant CAVA protein. b SDS-PAGE showing an eluent obtained from Ni–NTA column chromatography. Lane 1 is flow through, lane 2 is washing with 20 mM imidazole and lanes 3 to 9 are eluent fractions. c Elution profile of ion-exchange chromatography. d SDS-PAGE of purified CAVA. Lane 1 is a molecular mass marker and lane 2 shows purified CAVA
