Table 2.

Purification summary

S. no.	Purification step	Volume (ml)	Protein (mg/ml)b	Total protein (mg)	Yield (100 %)
1	E. coli cell pelleta	60	43.91	2635.02	100
2	Supernatant	56	1.428	79.968	3.034
3	Ni-affinity chromatography	15	0.136	2.04	0.077
4	Weak anion chromatography (DEAE column)	2	0.504	1.008	0.038

^aFrom 8 g of wet weight E. coli cell pellet (from 4 litre of batch)

^bProtein concentration determined by Lowry assay using BSA as a standard protein