Figure 5.

Biochemical characterization of the catalase activity of the purified enzyme. (A) Optimal temperature of the catalase activity (CAT) of the purified enzyme toward H₂O₂. (B) Optimal pH of the CAT toward H₂O₂. (C) Effects of inhibitors (final concentration, 1 mM) on the CAT toward H₂O₂. The residual CAT was measured by spectrophotometer in the standard reaction mixture with or without (CK) inhibitor (at 1 mM final concentration). Data are from three independent experiments (mean ± SD). Statistical significance was analyzed by Student's t-test with the control; ^***P < 0.001; ns, not significant.