Table 1.
Primer name | Primer sequence (5′ → 3′) | Purpose |
---|---|---|
AcCATPOF1 | ATGGATCCATACAAGTACCGAC | Cloning cDNA of the purified enzyme |
AcCATPOR1 | CTTCACATGGTTGGCCTCAC | |
3′ GSP I | CCATCAAGATTTGATCCAGTTCGTGAGGCAG | |
3′ GSP II | CAAGGAACCAGGGGAGAGATACAG | |
5′ GSP I | AGAACACCGGGAAGTTGTTACCTACTATG | |
5′ GSP II | AGCACTTGCACCTCTAGCATG | |
AcCATPO-F | ATGGATCCTTACAAGTATCGGCCTTCAAGTG | |
AcCATPO-R | TTCACATGGTTGGTCTTATGTTAAGTC | |
qAt-Actin-1F | CGTGACCTGACTGATTACCCTA | qRT-PCR |
qAt-Actin-1R | ACCTCAGGGCAACGGAAT | |
q-AcCATPO-1F | CCCCAGAGGTCCGATTC | |
q-AcCATPO-1R | CGCAAGTGAGATGGGAAACG |