Table 2.

Purification of AcCATPO from leaves of red amaranth.

Purification step	Volume (mL)	Total activity (U)			Total protein (mg)	Specific activity (U/mg)			Yield (%)			Purification fold
		CAT	MO	DO		CAT	MO	DO	CAT	MO	DO	CAT	MO	DO
Crude extract	40	1150	0.082	0.9448	35.65	32.3	0.0023	0.0265	100	100	100	1	1	1
Gel slice	46	116	0.0089	0.101	0.5	230.7	0.0177	0.2011	10.1	10.8	10.7	7.2	7.7	7.6

CAT, catalase activity; MO, monophenolase activity; DO, diphenolase activity. Each specific activity was measured under its optimal conditions. Optimal Temperature 60°C and pH 8.0 for MO and DO; Optimal Temperature 20°C and pH 7.0 for CAT.