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Kinetic properties of the purified enzyme, AcCATPO.
| AcCATPO | Optimal T (°C) | Optimal pH | Kmvalue (mM) | Vmax (μM product/min) | [ET] (μM) | Kcat (S−1) | Kcat/Km (M−1 S−1) |
|---|---|---|---|---|---|---|---|
| MO | 60 | 8.0 | 0.2 | 0.079 | 0.0345 | 0.04 | 200 |
| DO | 60 | 8.0 | 0.6 | 0.75 | 0.0345 | 0.36 | 600 |
| CAT | 20 | 7.0 | 188 | 5942 | 0.0345 | 2871 | 15271 |
MO, monophenolase activity to L-tyrosine; DO, diphenolase activity toward L-DOPA; CAT, catalase activity toward H2O2; T, temperature.
