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. 2016 Jan 8;5:294. doi: 10.3389/fonc.2015.00294

Figure 1.

Figure 1

Detection of apoptosis in situ and in western blot in HSE and human skin explants 24 h after irradiation with high doses of X-ray and carbon ions. (A) Immunodetection of active caspase-3 (brown) in HSE; cleaved caspase-3 was not detected in the epidermis after irradiation, but in the PCs (arrows in a); N = 3, n = 5. (B) Western blot analysis of caspase-3 and PARP in HSE; apoptosis is detected by the presence of caspase-3 and PARP cleavage fragments (17 and 19 kDa; 89 kDa) in PCs; active/cleaved caspase-3 and cleaved PARP were not detected in the epidermis of HSE after irradiation; N = 2, n = 4. (C) Immunodetection of cleaved caspase-3 (pink) in human skin explants; cleaved caspase-3 was not found after low or high doses of X-rays; *: melanocytes in the basal layer (brown); N = 3, n = 5.