FIGURE 2.

Lactose triggers expression of tapA and eps operons in B. subtilis. WT cells harboring P_tapA-cfp (YC189) or P_eps-gfp (YC164) were grown in LB or LB supplemented with 3% lactose. Cultures were then incubated for 5 h at 37°C and 50 rpm. A sample from each culture was then analyzed using a confocal microscope. The right picture are the bacteria taken using Nomarski differential interference contrast (DIC), at 40× magnification and the left pictures are the fluorescent bacteria. The top panel shows the expression of CFP and the bottom panel expression of GFP. Images are representative of five biological repeats.