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. 2016 Jan 8;6:1517. doi: 10.3389/fmicb.2015.01517

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Copyright © 2016 Duanis-Assaf, Steinberg, Chai and Shemesh.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lactose induces AI-2 secretion in B. subtilis. B. subtilis cells were grown in LB supplemented with 0–3% lactose. Cultures were then incubated for 5 h at 37°C and 50 rpm. A supernatant sample from each culture was taken and incubated with Vibrio harveyi MM77. Optical density and luminescence data were recorded every 30 min. (A) Bioluminescence kinetics using relative luminescence (RLU). The data are displayed as a mean value of results from two biological repeats each performed as triplicate. (-LB, 1% lactose, 2% lactose, ●3% lactose). (B) The area under the curve (AUC). The data were analyzed by means of ANOVA following post hoc t-test with Bonferroni correction. ^∗P-value < 0.01 compared to control.

Inline graphic — Lactose induces AI-2 secretion in B. subtilis. B. subtilis cells were grown in LB supplemented with 0–3% lactose. Cultures were then incubated for 5 h at 37°C and 50 rpm. A supernatant sample from each culture was taken and incubated with Vibrio harveyi MM77. Optical density and luminescence data were recorded every 30 min. (A) Bioluminescence kinetics using relative luminescence (RLU). The data are displayed as a mean value of results from two biological repeats each performed as triplicate. (-LB, 1% lactose, 2% lactose, ●3% lactose). (B) The area under the curve (AUC). The data were analyzed by means of ANOVA following post hoc t-test with Bonferroni correction. ^∗P-value < 0.01 compared to control.