Table 5.
Stability of the seven analytes in rat plasma (n = 3) under different storage conditions.
| Compounds and added concentration (ng/ml) | Freeze-thraw stability |
Long-term stability |
Short-term temperature stability |
Post-preparation stability |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Found concentration (ng/ml) | Precision RSD (%) | Accuracy RE (%) | Found concentration (ng/ml) | Precision RSD (%) | Accuracy RE (%) | Found concentration (ng/ml) | Precision RSD (%) | Accuracy RE (%) | Found concentration (ng/ml) | Precision RSD (%) | Accuracy RE (%) | |
| Quercetin | ||||||||||||
| 0.666 | 0.65 ± 0.05 | 7.70 | −1.90 | 0.64 ± 0.05 | 8.29 | −4.01 | 0.66 ± 0.04 | 5.29 | −0.40 | 0.68 ± 0.04 | 5.14 | 2.60 |
| 6.666 | 6.25 ± 0.10 | 1.60 | −6.19 | 6.28 ± 0.32 | 5.09 | −5.83 | 6.02 ± 0.07 | 1.11 | −9.64 | 5.94 ± 0.15 | 2.58 | −10.94 |
| 53.33 | 52.70 ± 1.59 | 3.02 | −1.19 | 51.59 ± 2.03 | 3.94 | −3.26 | 51.08 ± 2.00 | 3.91 | −4.22 | 51.38 ± 0.88 | 1.72 | −3.66 |
| Kaempferol | ||||||||||||
| 0.25 | 0.24 ± 0.01 | 5.53 | −2.07 | 0.24 ± 0.01 | 4.17 | −4.67 | 0.25 ± 0.02 | 6.51 | 0.53 | 0.25 ± 0.02 | 9.49 | −1.2 |
| 2.5 | 2.63 ± 0.08 | 3.08 | 5.12 | 2.66 ± 0.08 | 3.07 | 6.32 | 2.48 ± 0.10 | 4.13 | −0.88 | 2.48 ± 0.08 | 3.30 | −0.88 |
| 20 | 21.62 ± 0.82 | 3.81 | 8.09 | 19.04 ± 0.41 | 2.13 | −4.59 | 20.39 ± 0.50 | 2.47 | 1.93 | 20.57 ± 0.37 | 1.80 | 2.87 |
| Isorhamnetin | ||||||||||||
| 0.5 | 0.52 ± 0.01 | 1.12 | 3.33 | 0.47 ± 0.02 | 3.23 | −5.33 | 0.50 ± 0.02 | 4.19 | −0.67 | 0.51 ± 0.04 | 8.55 | 2.00 |
| 5 | 5.32 ± 0.02 | 0.29 | 6.33 | 5.07 ± 0.14 | 2.81 | 1.4 | 5.16 ± 0.28 | 5.44 | 3.13 | 5.00 ± 0.03 | 0.61 | 0.07 |
| 40 | 43.85 ± 1.23 | 2.81 | 9.63 | 39.51 ± 1.10 | 2.76 | −1.23 | 42.28 ± 1.08 | 2.54 | 6.20 | 43.15 ± 0.61 | 1.42 | 7.88 |
| Bilobalide | ||||||||||||
| 0.666 | 0.67 ± 0.03 | 3.86 | 0.64 | 0.67 ± 0.02 | 3.50 | −0.13 | 0.63 ± 0.03 | 4.52 | −5.88 | 0.61 ± 0.03 | 5.29 | −8 |
| 6.666 | 6.56 ± 0.19 | 2.84 | −1.55 | 7.10 ± 0.21 | 2.95 | 6.52 | 6.17 ± 0.22 | 3.62 | −7.4 | 6.08 ± 0.14 | 2.37 | −8.75 |
| 53.33 | 54.64 ± 1.07 | 1.97 | 2.45 | 54.46 ± 2.88 | 5.29 | 2.11 | 51.47 ± 0.97 | 1.88 | −3.49 | 53.02 ± 0.92 | 1.74 | −0.59 |
| Ginkgolide A | ||||||||||||
| 0.5 | 0.46 ± 0.04 | 9.48 | −8 | 0.49 ± 0.01 | 1.53 | −2.07 | 0.46 ± 0.03 | 6.94 | −7.33 | 0.55 ± 0.02 | 2.76 | 10.67 |
| 5 | 4.91 ± 0.07 | 1.50 | −1.9 | 5.07 ± 0.16 | 3.11 | 1.34 | 5.01 ± 0.07 | 1.40 | 0.14 | 4.92 ± 0.14 | 2.76 | −1.54 |
| 40 | 42.30 ± 0.17 | 0.39 | 5.76 | 41.84 ± 1.77 | 4.23 | 4.61 | 41.64 ± 0.85 | 2.04 | 4.11 | 42.02 ± 0.70 | 1.67 | 5.06 |
| Ginkgolide B | ||||||||||||
| 0.833 | 0.85 ± 0.05 | 6.19 | 1.82 | 0.85 ± 0.04 | 4.35 | 1.68 | 0.87 ± 0.02 | 2.45 | 4.76 | 0.82 ± 0.01 | 1.52 | −1.44 |
| 8.333 | 8.26 ± 0.18 | 2.13 | −0.93 | 8.46 ± 0.29 | 3.49 | 1.51 | 8.50 ± 0.14 | 1.60 | 2.05 | 8.01 ± 0.21 | 2.67 | −3.84 |
| 66.66 | 65.09 ± 1.29 | 1.98 | −2.36 | 63.37 ± 2.82 | 4.46 | −4.93 | 67.04 ± 1.76 | 2.62 | 0.58 | 65.74 ± 0.31 | 0.47 | −1.38 |
| Ginkgolide C | ||||||||||||
| 0.833 | 0.76 ± 0.08 | 9.92 | −8.36 | 0.75 ± 0.03 | 3.87 | −10.36 | 0.80 ± 0.01 | 1.25 | −3.96 | 0.88 ± 0.05 | 5.85 | 5.24 |
| 8.333 | 8.56 ± 0.32 | 3.74 | 2.68 | 8.54 ± 0.51 | 5.92 | 2.44 | 8.41 ± 0.29 | 3.40 | 0.92 | 7.95 ± 0.23 | 2.95 | −4.60 |
| 66.66 | 71.95 ± 0.74 | 1.03 | 7.93 | 65.80 ± 2.61 | 3.97 | −1.29 | 70.16 ± 1.88 | 2.67 | 5.25 | 67.21 ± 0.27 | 0.40 | 0.83 |