FIGURE 2.

A time course of Gp9 accumulation levels in Nicotiana benthamiana. (A) Immunoblots of TSP extracted from N. benthamiana leaf tissue infiltrated with gp9 constructs targeting the ER, the chloroplasts, or the ER fused to an ELP tag (ER-ELP) or fused to a hydrophobin tag (ER-HFBI). Four plants were infiltrated with each construct. Tissue was sampled from infiltrated leaves from 3 to 6 dpi. Equal volumes of TSP from each of the four replicates were pooled, and 20 μg was loaded per well. Immunoblots were probed with (A) an anti-c-Myc antibody or (B) an anti-Gp9 antibody. p19: TSP from plants infiltrated with p19 serving as a negative control.