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. 2016 Jan 8;6:1221. doi: 10.3389/fpls.2015.01221

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Copyright © 2016 Miletic, Simpson, Szymanski, Deyholos and Menassa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantification of Gp9 accumulation in N. benthamiana over 3, 4, 5, and 6 dpi. Quantification was performed on TSP extracts from infiltrated N. benthamiana tissue using a standard curve of known amounts of purified Gp9-ELP. Gp9 was targeted to the ER, the chloroplasts, or the ER fused to an ELP tag (ER-ELP) or fused to a hydrophobin tag (ER-HFBI). Immunoblots were probed with an anti-Gp9 antibody. Accumulation levels of Gp9 are shown in μg per g of FLW. Error bars represent the standard error of the mean value of four biological replicates. Treatments which do not share a letter are significantly different (P < 0.05) as determined by a one way ANOVA and the Tukey test.