FIGURE 4.

Purified Gp9-ELP is found in higher molecular weight protein complexes. Purified Gp9-ELP was electrophoresed under the following conditions: in sample buffer containing DTT and heat-denatured by boiling for 10 min, in sample buffer containing DTT with no heat denaturation, or in sample buffer without DTT and no heat denaturation. The plant extract containing Gp9-ELP was also loaded as a positive control in sample buffer containing DTT and heat-denatured by boiling for 10 min. Twenty microliters of extract from plants infiltrated with p19 were used as a negative control. Gels were either coomassie stained (A) or immunodetected (B) using an anti-c-Myc antibody.