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. 2016 Jan 8;6:1221. doi: 10.3389/fpls.2015.01221

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Copyright © 2016 Miletic, Simpson, Szymanski, Deyholos and Menassa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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S. Typhimurium binding activity to plant produced Gp9-ELP. (A) The Escherichia coli produced His₆-Gp9 (62 kDa) and the plant-produced Gp9-ELP (82 kDa) both react with anti-Gp9 antibodies. (B) Either 500 ng of His₆-Gp9, BSA, or Gp9-ELP protein were spotted onto nitrocellulose and then probed with 10⁸ cfu/ml GFP-expressing S. Typhimurium and allowed to grow overnight. Two biological replicates were done, each with three technical replicates in each. One representative image and densitometry analysis is shown.