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. 2016 Jan 8;6:1546. doi: 10.3389/fmicb.2015.01546

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Copyright © 2016 Wise and Binns.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The null phenotype of AB400 (virAΔR on pTi) can be overcome by constitutive expression of either virAΔR or virG. AB400/pSW209Ω (virB-lacZ) carrying pAW10 (vector), pYW47 (P_N25-virG) or pAW117 (P_N25-virAΔR) were assayed for vir gene expression. (A) AB^∗ induction medium included glycerol as the carbon source and 0, 30, or 100 μM AS. Student’s t-test indicates that pAW47 and pAW117 containing strains are different from one another (p > 0.95). (B) Induction medium included glucose in addition to glycerol and 0, 3, or 100 μM AS.