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. 2016 Jan 8;6:1546. doi: 10.3389/fmicb.2015.01546

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Copyright © 2016 Wise and Binns.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Glucose dose response assays indicate that the ChvE-dependent sugar signal affects a function of VirA that is partly independent from the effect of the phenolic inducer. A136 (no pTi) carrying either pAW100 (wt virA, P_N25-virG, virB-lacZ) or pAW97 (virAΔR, P_N25-virG, virB-lacZ) were assayed in AB^∗ induction medium with increasing concentrations of glucose. (A) Induction media did not contain AS. (B) Induction media contained 1 μM AS. (C) Induction media contained 10 μM AS.