Table 2.
Effect of different concentrations of PGRs on the regeneration frequencies of calli on MS media.
| Treatment | PGR (mg L−1) |
No of calluses inoculated | Regeneration frequency (%) | |||
|---|---|---|---|---|---|---|
| BAP | Kinetin | NAA | TDZ | |||
| RM1 | 0.5 | 0.5 | 0.5 | 0 | 12 | 33.3 ± 14.43ab |
| RM2 | 0.5 | 0.5 | 0.5 | 0.5 | 12 | 66.6 ± 38.18ab |
| RM3 | 0.5 | 1.0 | 0.5 | 0.5 | 12 | 93.3 ± 11.54b |
| RM4 | 0.5 | 1.5 | 0.5 | 0.5 | 12 | 100 ± 0.00b |
| RM5 | 0.5 | 0.5 | 1.0 | 0.5 | 12 | 53.3 ± 50.33ab |
| RM6 | 0.5 | 0.5 | 1.5 | 0.5 | 12 | 40 ± 52.91ab |
| RM7 | 1.5 | 0.5 | 0.5 | 0.5 | 12 | 91.6 ± 14.43ab |
Values are means of replicates ± SD. Means on the same column with the different superscripts are significantly different (P < 0.05). RM is defined as regeneration media. Total cytokinin refers to total amount of BAP + Kin concentration. BAP: benzylaminopurine; NAA: naphthaleneacetic acid; TDZ: thidiazuron. The bold values show the highest frequencies of plant regeneration.