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The grf10Δ mutant is delayed in germ-tube formation. Strains BWP17 (parental wild-type) and RAC117 (grf10Δ) were inoculated in (A) 10% fetal calf serum, (B) M199 medium, and (C) Spider medium, and were grown at 37°C; aliquots were removed microscopy analysis at 0, 4, 8 and 24 h. Cellular morphology was examined at 40× magnification. Note—the photos of the cells at time 0 are the same, and are reproduced here for ease of comparison across the time course.
