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. 2011 Nov 24;22(11):2486–2494. doi: 10.1093/cercor/bhr326

Figure 2.

Figure 2.

Effects of intra-mPFC DA D4 receptor and CaMKII inhibition during fear-conditioning. (A) Saline controls displayed no significant difference in time spent freezing in response to CS+ vs. CS− presentations following subthreshold (0.4 mA; see Materials and Methods) fear conditioning. In contrast, intra-PLC PD 168077 (50 ng/0.5 μL) significantly potentiated the acquisition of subthreshold fear memory. Coadministration with the CaMKII inhibitor KN-62 (2.5–250 ng/0.5 μL dose dependently attenuates the effects of D4 receptor activation. In contrast, intra-PLC KN-62 (250 ng/0.5 μL) alone did not have a significant effect on the acquisition of suprathreshold (0.8 mA) fear memory. (B) Analysis of exploratory scores revealed that saline controls showed no CS+-related exploratory behavior suppression. In contrast, PD 168077 (50 ng/0.5 μL) significantly potentiated CS+-related suppression of exploratory behavior, which was dose dependently blocked by coadministration of KN-62 (2.5–250 ng/0.5 μL). Bars represent mean ± standard error of the mean for this and all subsequent figures.