TABLE 1.
Plasmids used in this work
| Plasmid | Description | Reference |
|---|---|---|
| pUC19 | E. coli cloning vector, AmpR, MCS | Ref. 29 |
| pUCB2 | pUC19 /pUB110 shuttle vector, NeoR, PhleoR, AmpR | Brantl (unpublished observations) |
| pUCBE1 | pUCB2 with bsrE gene under own promoter and 96 bp upstream of − 35 box of pbsrE | Footnote 4 |
| pUCB5.1 | pUCB2 with sr5 gene under own promoter and 87 bp upstream of − 35 box of psr5 | Footnote 4 |
| pUCBES2 | pUCB5.1 with bsrE gene under own promoter and 96 bp upstream of − 35 box | Footnote 4 |
| pUCBES4 | As pUCBES2, nt 51–163 of SR5 are lacking | This study |
| pUCBES6 | As pUCBES2, SR5 loop sequences of SL2 and SL4 exchanged by heterologous sequences | This study |
| pUCBES7 | As pUCBES2, nt 1–47, 91–104, and 126–163 of SR5 are lacking | This study |
| pUCBES8 | As pUCBES2, nt 49–78 of SR5 are lacking | This study |
| pUCBES9 | As pUCBES2, nt 1–46 and 105–163 of SR5 are lacking | This study |