FIGURE 6.

Deficiency of Arrb2 inhibits hepatocyte apoptosis through Fas. A, WT and Arrb2 KO mice were administered Jo2 at two different doses (0.15 and 0.3 μg/g body weight, i.p.). After 6 h of treatment, livers were harvested and fixed in 10% buffered formalin. Apoptotic cells (dark cells) were examined by TUNEL assay. **, p < 0.01; ***, p < 0.001. B, mice were injected as in A. At 6 h of injection, the expression of total Akt and p-Akt in livers was examined by immunoblot. C, WT and Arrb2 KO MEFs were transfected with Arrb2 full-length vector or control (Con) vector. After transfection for 24 h, the cells were treated with Jo2 (200 ng/ml), and the levels of total Akt and p-Akt were measured by Western blot. *, p < 0.01. D, WT, Arrb1 KO, Arrb2 KO, and Arrb1/2 double KO MEFs were treated with Jo2 (200 ng/ml) for 24 h. Apoptosis was determined by TUNEL assay. *, p < 0.05; **, p < 0.01.