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Enzyme kinetics for loading of UBL variants on UBE2Z. A, Michaelis-Menten fits for E2-thioester formation assays are shown for ubiquitin (red), FAT10 (blue), and UbCYCI (purple) on UBE2Z and for FAT10LRLR and on UBE2Z (green). B, the expanded view of fits for FAT10 and UbCYCI loading onto UBE2Z are shown in the same colors as previously. C, histogram summarizing kinetic parameters apparent Km and apparent Vmax for the loading of different UBL and UBL mutants onto UBE2Z variants.
