FIGURE 1.

PD174265 induced GPR17 expression in Oli-neu cells. Cells were cultured in Sato medium either untreated (A) or in the presence of 1 μm PD174265 (B) for 48 h. Note the induced formation of oligodendrocytic processes in treated Oli-neu cells. Scale bar = 20 μm. C, lysates from Oli-neu cells cultivated in the absence or presence of 1 μm PD174265 after 24, 48, and 72 h were analyzed by Western blotting using the GPR17 antibody (upper panel) and, after stripping, reprobed with the β-actin antibody (lower panel). D, quantitative analysis of GPR17 immunoreactive band corrected by β-actin from three independent experiments.