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. 2015 Nov 30;291(2):705–718. doi: 10.1074/jbc.M115.683953

FIGURE 3.

FIGURE 3.

GPR17 activation modified oligodendroglial cell global activity. A, representative traces of MDL29,951 determined by label-free DMR assays in Oli-neu cells pretreated for 24 h with 1 μm PD174265. B, concentration-effect curve derived from the DMR traces in A of seven independent experiments; DMR responses were normalized for each experiment as the percentage of the values obtained with 100 μm MDL29,951. C, characteristic DMR recordings of MDL29,951 in primary rat differentiating oligodendrocytes after culturing for 48 h in growth factor-free medium. D, DMR dose-effect curve in oligodendrocytes from the traces of six independent experiments; DMR responses were normalized for each experiment to the values obtained with 30 μm MDL29,951. E and F, representative DMR assays in Oli-neu cells after transfection with scrambled siRNA (E) and GPR17-siRNA (F); note the decrease in MDL29,951-induced traces upon GPR17 knockdown. G, concentration-response quantification of DMR responses in Oli-neu cells after GPR17 knockdown from five independent experiments. siSc, scrambled siRNA; siGPR17, GPR17-siRNA. H, GPR17-siRNA knockdown does not impair whole-cell DMR recordings triggered by 100 μm ATP in Oli-neu cells (n = 5); DMR responses were normalized for each experiment to the values obtained with 30 μm MDL29,951 (MDL) in Oli-neu cells after transfection with scrambled siRNA. I, illustrative Western blot (upper panel) and quantitative analysis from five independent experiments (lower panel) of GPR17 expression levels upon GPR17 knockdown in primary rat oligodendrocytes. ***, p < 0.001. J, representative DMR assays in rat oligodendrocytes after transfection with scrambled siRNA or GPR17-siRNA. K, dose-response quantification of DMR responses in oligodendrocytes after GPR17 knockdown from six different experiments. L, DMR responses triggered by 100 μm ATP are not affected by GPR17 knockdown in differentiating oligodendrocytes (n = 6); DMR responses were normalized for each experiment to the values obtained with 100 μm MDL29,951 (MDL) in rat oligodendrocytes after transfection with scrambled siRNA.