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. 2015 Nov 30;291(2):705–718. doi: 10.1074/jbc.M115.683953

FIGURE 9.

FIGURE 9.

Activation of EPAC by treatment with 8-CPT-2′-O-Me-cAMP promoted oligodendrocyte differentiation in the presence of GPR17 agonist. A, rat oligodendrocytes were differentiated in the presence of triiodothyronine (T3) for 24, 48, and 72 h; lysates were analyzed by Western blotting for EPAC expression with antibodies recognizing EPAC1/2 (EPAC). B, assessment of EPAC abundance during oligodendrocyte differentiation from four different experiments. C, upper panel, representative Western blotting demonstrating that co-treatment with the EPAC activator 8-CPT-2′-O-Me-cAMP (EPAC-A) increases MBP expression in the presence of 30 μm MDL29,951 in rat oligodendrocytes differentiated with T3 for 48 h. Lower panel, quantitative analysis from six independent experiments of MBP expression levels in primary rat oligodendrocytes. D, upper panel, illustrative Western blot displaying MBP expression levels in Oli-neu cells differentiated with PD174265 for 48 h treated with 10 μm MDL29,951 and 8-CPT-2′-O-Me-cAMP. Lower panel, bar graph showing quantification of MBP abundance in Oli-neu cells from eight different experiments. *, p < 0.05; **, p < 0.01.