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. 2015 Nov 15;291(2):719–730. doi: 10.1074/jbc.M115.686006

FIGURE 4.

FIGURE 4.

Effect of miRNA overexpression on PLD protein expression and activity. A–B, PLD2 protein expression in MCF-7 and MDA-MB-231 cells, respectively, in the presence of miR overexpression was detected by Western blot (W.B.). β-Actin was used as a control. C, PLD2 protein expression was decreased with increasing amounts of overexpressed microRNA-3619. β-Actin was used as a control. D, PLD enzymatic activity was measured in HMEC, MCF-7, and MDA-MB-231 cell lines in the presence of microRNA overexpression. Non-transfected cells were used as a control. The symbols * or # denote statistically significant (p < 0.05) increases or decreases, respectively, between samples and controls. E, effect of different combination of miRs on PLD enzymatic activity.