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. 2015 Nov 15;291(2):719–730. doi: 10.1074/jbc.M115.686006

FIGURE 5.

FIGURE 5.

Overexpression of miR plasmids abrogated PLD protein expression in cells resulting in decreased cell invasion. A, cells were overexpressed with GFP-tagged miR plasmids. Immunofluorescence indicates decreased PLD protein expression in the presence of miR overexpression. Bars = 5 μm. B, Matrigel invasion assay showed increased cell invasion with PLD overexpression. Invasion returned to basal level with the addition of PLD-targeting miRNA. C, Matrigel invasion assay on cells previously transfected with siRNAs specific for the indicated miRs. D and E, overexpression of select miR plasmids or mimics, respectively, decreased cell invasion in MCF-7 and MDA-MB-231 cells. F, representative images of cell invasion assay showing a decrease in invaded cell in the presence of miR-203 using 10× magnification. In B–E, * and # represent significance (p < 0.05) by ANOVA over the respective controls, either increases (*) or decreases (#).