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. 2015 Nov 2;291(2):773–789. doi: 10.1074/jbc.M115.694919

FIGURE 2.

FIGURE 2.

Knockdown of Rab10 inhibits exocytosis of Glut4 in adipocytes. A, 3T3-L1 adipocytes expressing HA-Glut4/GFP and shRNA (control, white; Rab10, gray) were pretreated ± 100 nm insulin for 45 min and then incubated at 37 °C for increasing times with AF647-α-HA ± insulin. Circles, basal; squares, insulin. Data are standardized to Ymax control insulin and are the average MFR ± S.D. (uptake) of n = 4–9 experiments (Table 1). Lines, single-exponential fits of the data (kobs = kex; Ymax = total cycling pool size). B, kex ± S.D. determined from single exponential fits of the combined data. ***, p ≤ 0.0001, that control and Rab10 knockdown cells are best fit by the same function.