FIGURE 4.
Knockdown of Rab10 has no effect on Glut4 trafficking in fibroblasts. Fibroblasts expressing HA-Glut4/GFP and shRNA (control, white; Rab10, gray) were treated as described in Figs. 1–3, and surface HA-Glut4/GFP was labeled. A, basal to insulin transition, average MFR ± S.D. of n = 4–10 independent experiments. Lines, simulations (Table 3). B, total HA-Glut4/GFP expression (GFP), mean ± S.D. of n = 64 samples from two independent experiments. ***, p ≤ 0.0001, and values are the same in control and knockdown cells. C, ken ± S.D. determined from single-exponential decay fits of D. Insulin + LYi transition data average MFR ± S.D. of n = 5–6 independent experiments. Lines, single exponential fits of the data. All data are standardized to control samples as indicated.