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. 2015 Nov 11;291(2):848–861. doi: 10.1074/jbc.M115.685271

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — DNA templates for in vitro transcription were characterized following synthesis. A, I-PpoI digestion of DNA templates for in vitro transcription. Lane M, New England Biolabs 50-bp DNA ladder labeled with [³²P]phosphate; lane 1, I-PpoI digestion of unmodified control DNA template; lane 2, I-PpoI digestion of B[a]P-N⁶-dA-modified DNA template. B, BsiWI digestion of DNA templates for in vitro transcription. Lane M, IDT 20/100 DNA ladder labeled with [³²P]phosphate; lane 1, BsiWI digestion of unmodified control DNA template; lane 2, BsiWI digestion of B[a]P-N⁶-dA-modified DNA template.