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Site-specifically modified vectors for transcription in cells were characterized. The presence of either B[a]P-N6-dA (A) or B[c]Ph-N6-dA adducts (B) within a BsiWI restriction sight located in the vector prevents BsiWI digestion. Vectors were incubated with the restriction enzymes shown, and the products were resolved by agarose gel electrophoresis.
