TABLE 2.

Primers for qRT-PCR-mediated quantification of RFP and GFP mRNA

Oligomer	Sequence
Anchored poly(dT) RT primer	5′-ATGTTGACGCAGCCAGTGACT20VN-3′a
GFP-cDNA-FWD	5′-TAGTTGCCAGCCATCTGTTG-3′
GFP-cDNA-PROBE	5′-FAM-AAGGTGCCA/ZEN/CTCCCACTGTCCTTT-IBFQ-3′b
cDNA-REV	5′-ATGTTGACGCAGCCAGTGAC-3′
RFP-cDNA-FWD	5′-GAGGCTAACTGAAACACGGA-3′
RFP-cDNA-PROBE	5′-FAM-AGGAGACAA/ZEN/TACCGGAAGGAACCC-IBFQ-3′b

^a V represents A, C, or G; N represents A, C, G, or T.

^b FAM, 6-FAM^TM; ZEN, ZEN^TM internal quencher; IBFQ, Iowa Black® FQ.