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Summary of the kinetic scheme determined using in proteo MS and conventional time-resolved and stopped-flow UV/vis approaches. Approximate rate constants measured by each of these methods are given. Standard conditions used to obtain all first-order rate constants were 10 μm IsdG-heme, 10 mm ascorbate, 50 mm KPi, pH 7.4, 22 °C, 100 units of catalase.
