FIGURE 2.

Gap junctional inhibitors 18a-GA and oleamide inhibited SMG branching morphogenesis. A, phase contrast image of SMG organ cultures with and without 18α-GA. B, quantification of data shown in A, with the numbers of terminal buds at 24 and 48 h compared with those at 2 h. C, quantification of number of terminal buds in presence of PDGF-AA, PDGF-BB, FGF-7, or FGF-10 with or without 18α-GA (2–5 μm). Statistical analysis was performed using ANOVA (n = 6 per group; *, p < 0.01; **, p < 0.05).