FGF10 induced ERK1/2 phosphorylation in salivary gland epithelial cell line HSY.
A, ERK1/2 phosphorylation in human salivary epithelial cell line HSY after stimulation with FGF10 with or without 18α-GA was analyzed by western immunoblotting. B, quantitation of findings shown in A. C, phosphorylation of ERK1/2 in HSY cells after stimulation with FGF10 for 5 min with 18α-GA, Cx43 peptide (Cx43 pep), or siRNA for Cx43 (siCx43) was analyzed by Western immunoblotting. D, BrdU incorporation in HSY cells after stimulation with FGF10 with or without 18α-GA or MEK inhibitor U0126. E, intracellular calcium level [Ca2+]i was measured after stimulation of FGF10 with Cx43 peptide or 2-APB. Statistical analysis was performed using ANOVA (n = 5 per group; *, p < 0.01).