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Deubiquitin enzyme library was screened for γH2AX by in vivo deubiquitylation assay. A–C, 293 cells were transiently transfected with plasmids DNA expressing Flag-H2AX, HA-RNF8, His-UB, and different Flag-DUB. The whole cell extracts and the elution of Ni-NTA-agarose bead pulldown were assayed by Western blot analysis using antibodies against γH2AX, H2AX, Flag, and HA.
