FIGURE 1.

Characterization of CEACAM2 expression in the endocrine pancreas. A, Ceacam2 mRNA levels were determined in triplicate in FACS-purified β-cells and non β-cells by qRT-PCR (n = 5 mice), normalized to β-actin (panel i), and measured relative to Ceacam1 mRNA (panel ii). Values were expressed as means ± S.E. *, p < 0.05 versus β-cells. B, liver tissues were removed from 5-month-old mice (n >3/genotype) and lysates were analyzed by 7% SDS-PAGE with sequential immunoblotting (Ib) with polyclonal antibody against CEACAM1 followed by re-immunoblotting (ReIb) with α-tubulin antibody for protein normalization. C, RNA was extracted from pancreatic islets of these mice and reverse-transcribed using specific Ceacam1 and Ceacam2 primers, followed by PCR amplification and analysis by 1% agarose gel. Gels in B and C represent at least two independent experiments.