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. 2015 Sep 21;51(1):110–118. doi: 10.1038/bmt.2015.215

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Flow cytometry for Tregs. PBMCs were stained with anti-CD3 PE-Cy7, -CD4 FITC, -CD8 APC-AF750, -CD25 PE, -FOXP3 APC, -CD127 PerCP-Cy5.5 and analysed on an LSRII Flow Cytometer (BD Biosciences, Oxford, UK). CD3⁺ cells were gated by CD3/FSC/SSC; CD3⁺CD4⁺CD8⁻ cells analysed for FOXP3 and CD25 expression; CD3⁺CD4⁺CD25⁺FOXP3⁺ cells analysed for expression of CD127. FSC, forward scatter; SSC, side scatter; Tregs, regulatory T cells.