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Evaluation of intracellular growth based on a growth reporter strain. A V. cholerae WT strain harboring a chromosomally encoded growth reporter construct was cocultured with A. castellanii. Growth of bacteria expressing destabilized GFP was detected in the CVs of trophozoites (upper row), the CVs of cysts (middle row) and the cytosol of cysts (lower row). Scale bar valid for all panels: 10 μm.
