Table 1. V. cholerae strains and plasmids used in this study.
| Genotype/descriptiona | Reference | |
|---|---|---|
| Strains | ||
| A1552 (WT) | Wild-type, O1 El Tor Inaba, RifR | Yildiz and Schoolnik (1998) |
| A1552-GFP | A1552 with mTn7-gfp; RifR, GentR | Blokesch (2012b) |
| A1552-dsRed | A1552 with mTn7-dsRed.T3[DNT]; RifR, GentR | Borgeaud et al. (2015) |
| A1552-TnrrnBP1-gfp(ASV) | A1552 with mTn7-rrnBP1-gfp(ASV); RifR, GentR; growth reporter strain | This study |
| ΔhapR | A1552 deleted for hapR (TransFLP); RifR | Borgeaud et al. (2015) |
| ΔhapR-GFP | ΔhapR with mTn7-gfp; RifR, GentR | This study |
| ΔhapR-dsRed | ΔhapR with mTn7-dsRed.T3[DNT]; RifR, GentR | This study |
| ΔhapRΔvpsA | A1552 deleted for hapR and vpsA (TransFLP); RifR | This study |
| ΔhapRΔvpsA-GFP | ΔhapRΔvpsA with mTn7-gfp; RifR, GentR | This study |
| ΔhapRΔvpsA-dsRed | ΔhapRΔvpsA with mTn7-dsRed.T3[DNT]; RifR, GentR | This study |
| ΔvpsA | A1552 deleted for vpsA (TransFLP); RifR | This study |
| ΔvpsA-GFP | ΔvpsA with mTn7-gfp; RifR, GentR | This study |
| Plasmids | ||
| pUX-BF13 | oriR6K, helper plasmid with Tn7 transposition function; AmpR | Bao et al. (1991) |
| pGP704::Tn7-GFP | pGP704 with mini-Tn7 harboring a constitutively expressed gfp cassette; AmpR | Müller et al. (2007) |
| pGP704-mTn7-dsRed | pGP704 with mini-Tn7 carrying dsRed.T3[DNT]; AmpR | Borgeaud et al. (2015) |
| pGP704Sac28-mTn7- rrnBP1-gfp(ASV) | pGP704 with mini-Tn7 carrying growth reporter rrnBP1-gfp(ASV); AmpR | This study |
a
TransFLP method according to De Souza Silva and Blokesch (2010) and Blokesch (2012a).