Table 2. Mixed color CV colonization assay.
| Inoculum GFP:dsRed=1:1 |
Colonized CV |
|||
|---|---|---|---|---|
| Mixed colors | Mixed colors | GFP only | dsRed only | |
| Expected (%)a | 100% | 100% | 0% | 0% |
| Observed (%)b | 100% | 79.7% (±5.5) | 11.7% (±4.1) | 8.6% (±1.6) |
| Supplementary Figure S4A | Supplementary Figure S4B | |||
a
Expected percentage values are based on the assumption of repeated vacuolar fusions delivering V. cholerae to the CV or single fusion events between one food vacuole containing many bacteria with the CV (e.g., no growth within the CV).
b
Averages are derived from three independent biological experiments (±s.d.).