Figure 7. Effect of DNA methylation inhibitor (MTA) on MGF-C25E-promoted DNA methylation.
(A) The relative levels of 5meCyt. Tenocytes were exposed to MGF-C25E (30 ng/mL) in the absence or the presence of MTA (0.05 μM) for 24 h, after which the methylation level was detected by immunostaining. Bars = 50 μm. (B) Quantitative analysis of the relative levels of 5meCyte in tenocytes. (C) The MTT assay shows that MTA at 0.05 μM has no effect on tenocyte viability within 24 h. The data are expressed as the means ± SD; n = 3 in each group; *p < 0.05 and **p < 0.01.